Biological Sample Disruption | TissueLyser ...

Biological Sample Disruption | TissueLyser II

Supplier: QIagen Chadstone

The TissueLyser II simultaneously disrupts multiple biological samples through high-speed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads.

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The TissueLyser II simultaneously disrupts multiple biological samples through high-speed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads. Using the appropriate adapter set, up to 48 or 192 samples can be processed at the same time. Alternatively, a grinding jar set can be used to process large samples. A range of beads, bead dispensers, and collection microtubes and caps are also available. All accessories for the TissueLyser II, including adapter sets, are also compatible with the TissueLyser.

Features

For medium- to high-throughput sample disruption for molecular analysis
Convenient and secure disruption process
Adapter sets optimized for high-throughput disruption
Wide range of accessories available
Reproducible results with difficult-to-lyse tissues
Front-end solution for QIAGEN automation

Procedure

TissueLyser Adapter Sets allow processing of up to 2 x 96 samples in collection microtubes or up to 2 x 24 samples in 2 ml microcentrifuge tubes.
The adapter sets can be precooled at –80°C if disrupting samples without lysis buffer.
Tubes remain securely sealed during disruption to prevent cross-contamination, which is especially important for highly sensitive downstream applications, such as real-time RT-PCR or microarray analysis.
Depending on the sample type, disruption is carried out using stainless steel, tungsten carbide, or glass beads.
TissueLyser Bead Dispensers are available to conveniently deliver single beads into microcentrifuge tubes or to deliver 96 beads in parallel into collection microtubes. The TissueLyser II can also disrupt large samples when used in combination with Grinding Jar Sets.

Applications

The ability to process up to 192 samples per run makes the TissueLyser II the ideal front-end solution to access biological information for genomics, transcriptomics, and proteomics applications.
For next-generation, high-throughput sequencing technologies, the TissueLyser II is the disruption instrument of choice.
A wide range of QIAGEN sample purification kits are compatible with the TissueLyser II, and sample purification can be performed manually or can be automated using the QIAcube, QIAsymphony SP, EZ1 Advanced, or BioRobot or BioSprint workstations.

Specifications

Features	Specifications
Disruption principle	High-speed shaking of samples in 1.2 ml collection tubes or 2 ml microcentrifuge tubes with stainless steel or glass beads
Features	Convenient and secure disruption process. Adapter sets optimized for high-throughput disruption. Wide range of accessories available (e.g.,grinding jar set to process large samples). Reproducible results with difficult-to-lyse tissues. Front-end solution for QIAGEN automation.
Kits compatible with instrument	All kits for purifying RNA/DNA/Protein
Protocol/main application on this intrument	Sample preparation/sample disruption
Technical data	100–120/220–240 V, 50/60Hz; variable speeds from 3 to 30 Hz (180–1800 oscillations/minute)
Technology	Bead Mill
Throughput	2 x 96 collection microtubes (1.2 ml) or 2 x 24 microcentrifuge tubes (2ml)
Typical run time	15 sec - 2 x 3 minutes at 15–30 Hz

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Features

For medium- to high-throughput sample disruption for molecular analysis
Convenient and secure disruption process
Adapter sets optimized for high-throughput disruption
Wide range of accessories available
Reproducible results with difficult-to-lyse tissues
Front-end solution for QIAGEN automation

Procedure

TissueLyser Adapter Sets allow processing of up to 2 x 96 samples in collection microtubes or up to 2 x 24 samples in 2 ml microcentrifuge tubes.
The adapter sets can be precooled at –80°C if disrupting samples without lysis buffer.
Tubes remain securely sealed during disruption to prevent cross-contamination, which is especially important for highly sensitive downstream applications, such as real-time RT-PCR or microarray analysis.
Depending on the sample type, disruption is carried out using stainless steel, tungsten carbide, or glass beads.
TissueLyser Bead Dispensers are available to conveniently deliver single beads into microcentrifuge tubes or to deliver 96 beads in parallel into collection microtubes. The TissueLyser II can also disrupt large samples when used in combination with Grinding Jar Sets.

Applications

The ability to process up to 192 samples per run makes the TissueLyser II the ideal front-end solution to access biological information for genomics, transcriptomics, and proteomics applications.
For next-generation, high-throughput sequencing technologies, the TissueLyser II is the disruption instrument of choice.
A wide range of QIAGEN sample purification kits are compatible with the TissueLyser II, and sample purification can be performed manually or can be automated using the QIAcube, QIAsymphony SP, EZ1 Advanced, or BioRobot or BioSprint workstations.

Specifications

Features	Specifications
Disruption principle	High-speed shaking of samples in 1.2 ml collection tubes or 2 ml microcentrifuge tubes with stainless steel or glass beads
Features	Convenient and secure disruption process. Adapter sets optimized for high-throughput disruption. Wide range of accessories available (e.g.,grinding jar set to process large samples). Reproducible results with difficult-to-lyse tissues. Front-end solution for QIAGEN automation.
Kits compatible with instrument	All kits for purifying RNA/DNA/Protein
Protocol/main application on this intrument	Sample preparation/sample disruption
Technical data	100–120/220–240 V, 50/60Hz; variable speeds from 3 to 30 Hz (180–1800 oscillations/minute)
Technology	Bead Mill
Throughput	2 x 96 collection microtubes (1.2 ml) or 2 x 24 microcentrifuge tubes (2ml)
Typical run time	15 sec - 2 x 3 minutes at 15–30 Hz